Coding

Part:BBa_K4739004:Design

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-09)


Hcp-Minihepcidin fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 487
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. Choose codons that are frequently used in the host organism to ensure efficient translation. 2. Optimize for codons corresponding to abundant tRNAs in the host to enhance translation speed. 3. Limit repetitive DNA sequences that may lead to recombination events and genomic instability.


Source

Sequence of the linker is retrieved from a research. The sequence of Hcp is retrieved from P. aeruginosa genome. The sequence of Minihepcidin is created from its amino acid sequence.

References

Jana, B., Keppel, K., & Salomon, D. (2021). Engineering a customizable antibacterial T6SS‐based platform in Vibrio natriegens. EMBO Reports. https://doi.org/10.15252/embr.202153681